VcMMAE - CAS:646502-53-6 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	VcMMAE
英文名称:	VcMMAE
别名:	mc-vc-PAB-MMAE
CAS No:	646502-53-6	分子式:	C₆₈H₁₀₅N₁₁O₁₅	分子量:	1316.63
CAS No:	646502-53-6
分子式:	C₆₈H₁₀₅N₁₁O₁₅
分子量:	1316.63

点击下载产品说明书(PDF)

V10096 VcMMAE ≥98% (psaitong)

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包装规格：

1mg 5mg 10mg 25mg in glass bottle

溶解性：

溶于DMSO（≥ 54 mg/mL）

产品描述：

基本信息

产品编号：	V10096
产品名称：	VcMMAE
CAS:	646502-53-6	储存条件	粉末	2-8℃	四年

分子式：	C68H105N11O15		溶于液体	-80℃	两年
分子量	1316.63
化学名：	Maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl monomethylauristatin E
Solubility (25°C)：
体外:	DMSO	100 mg/mL (75.95mM)
	Ethanol	100 mg/mL (75.95mM)
	Water	Insoluble
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	0.7595mL	3.7976mL	7.5951mL
5mM	0.1519mL	0.7595mL	1.5190mL
10mM	0.0760mL	0.3798mL	0.7595mL
50mM	0.0152mL	0.0760mL	0.1519mL

生物活性

产品描述	抗体偶联药物的一部分 (drug-linker conjugate for ADC)，具有抗癌活性，它由 MMAE (一种 tubulin 抑制剂) 和 Vc 连接而成。
靶点	Auristatin
体外研究	Monomethyl auristatin E (MMAE) is efficiently released from SGN-35 within CD30+ cancer cells and, due to its membrane permeability, is able to exert cytotoxic activity on bystander cells. MMAE sensitized colorectal and pancreatic cancer cells to IR in a schedule and dose dependent manner correlating with mitotic arrest. Radiosensitization is evidenced by decreased clonogenic survival and increased DNA double strand breaks in irradiated cells
体内研究	Monomethyl auristatin E (MMAE) in combination with IR results in tumor growth delay, tumor-targeted ACPP-cRGD-MMAE with IR produces a more robust and significantly prolonged tumor regression in xenograft models

推荐实验方法(仅供参考)

Cell Assay

Monomethyl auristatin E (MMAE, 5nM) and ionizing radiation (IR) treated cells are harvested and lysed in RIPA buffer with protease and phosphatase inhibitors. 30μg of lysate undergo electrophoresis using 4-12% Bis-Tris gels, transferred to PVDF membranes and incubated with indicated primary antibodies. Blots are developed by ECL.

Animal Administration

6-8 week old female athymic nu/nu mice are injected subcutaneously into thighs with 5×106 HCT-116 or PANC-1 cells in a 1:1 Matrigel and PBS solution. Mice are treated with IR or intravenous (IV) injection of ACPP-cRGD-MMAE (6 nmoles/day, 18nmoles total, i.v.), tumor tissue is harvested, formalin fixed and paraffin embedded followed by staining with indicated antibodies. The primary antibody is used at a 1:250 dilution and is visualized using DAB as a chromagen with the UltraMap system.

保存条件：

2-8℃ 充氮保存

UN码：

HazardClass：

危害声明：

安全说明：

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