中文名称: | T-00127_HEV1 | ||||
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英文名称: | T-00127_HEV1 | ||||
别名: | 3-(3,4-Dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine | ||||
CAS No: | 900874-91-1 | 分子式: | C22H29N5O3 | 分子量: | 411.5 |
CAS No: | 900874-91-1 | ||||
分子式: | C22H29N5O3 | ||||
分子量: | 411.5 |
基本信息
产品编号: |
T11485 |
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产品名称: |
T-00127_HEV1 |
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CAS: |
900874-91-1 |
储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
溶于液体 |
-80℃ |
6个月 |
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分子量: |
411.50 |
-20℃ |
1个月 |
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化学名: |
3-(3,4-Dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine |
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Solubility (25°C): |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.4301mL |
12.1507mL |
24.3013mL |
5mM |
0.4860mL |
2.4301mL |
4.8603mL |
10mM |
0.2430mL |
1.2151mL |
2.4301mL |
生物活性
产品描述 |
一种phosphatidylinositol 4-kinase III beta (PI4KB)抑制剂,其IC50 值为60nM。 |
靶点 |
PI4KB 60nM (IC50) |
体外研究 |
T-00127_HEV1 shows more potent anti-poliovirus (PV) activity (EC50 of 0.77μM) than other candidate compounds (EC50 of 1.7 to 4.7μM).GW5074 and T-00127_HEV1 almost completely inhibit PI4KB kinase activity at 10μM (3% and 5% of residual activity,respectively),in contrast to AN-12-H5 (108% of activity [no inhibition]). |
推荐实验方法(仅供参考)
激酶实验: |
The inhibitory effect of T-00127_HEV1 at a concentration of 10μM on in vitro cellular protein kinase activities is assessed by kinase profiling with an ATP concentration near the Km for each kinase.Inhibitory effects of GW5074,AN-12-H5,and T00127_HEV1 at a concentration of 10μM on in vitro PI kinase activities are assessed by the SelectScreen kinase profiling service with an ATP concentration of 10μM.For T-00127_HEV1,the 50% inhibitory concentration (IC50) for in vitro PI4KB activity is also measured with an ATP concentration of 10μM. |
细胞实验: |
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RD cells (1.0×104 cells per well in 100μL medium) in a 96-well plate are infected with PV1,EV71,or CVB3 at multiplicities of infection (MOI) of 10,1.0,and 0.1 at 37℃ for 1h in the absence of T-00127_HEV1. The cells are washed three times with 10% FCS-DMEM, followed by the addition of 100μL of 10% FCS-DMEM containing 10 or 0μM T-00127_HEV1.Cells are collected at 16h p.i.,and then viral RNA is extracted from the cells using a viral RNA purification kit.The number of copies of the viral genome is quantified using a real-time PCR system. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )