T-00127_HEV1 - CAS:900874-91-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	T-00127_HEV1
英文名称:	T-00127_HEV1
别名:	3-(3,4-Dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine
CAS No:	900874-91-1	分子式:	C₂₂H₂₉N₅O₃	分子量:	411.5
CAS No:	900874-91-1
分子式:	C₂₂H₂₉N₅O₃
分子量:	411.5

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T11485 T-00127_HEV1 ≥98% (psaitong)

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T-00127_HEV1
CAS No：900874-91-1
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包装规格：

1mg 5mg in glass bottle

溶解性：

溶于DMSO(50mg/mL超声）

产品描述：

基本信息

产品编号：	T11485
产品名称：	T-00127_HEV1
CAS:	900874-91-1	储存条件	粉末	-20℃	四年
分子式：	C22H29N5O3		溶于液体	-80℃	6个月
分子量：	411.50			-20℃	1个月
化学名：	3-(3,4-Dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine
Solubility (25°C)：
体外:	DMSO
	Ethanol
	Water
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.4301mL	12.1507mL	24.3013mL
5mM	0.4860mL	2.4301mL	4.8603mL
10mM	0.2430mL	1.2151mL	2.4301mL

生物活性

产品描述

一种phosphatidylinositol 4-kinase III beta (PI4KB)抑制剂，其IC50 值为60nM。

靶点

PI4KB 60nM (IC50)

体外研究

T-00127_HEV1 shows more potent anti-poliovirus (PV) activity (EC50 of 0.77μM) than other candidate compounds (EC50 of 1.7 to 4.7μM).GW5074 and T-00127_HEV1 almost completely inhibit PI4KB kinase activity at 10μM (3% and 5% of residual activity,respectively),in contrast to AN-12-H5 (108% of activity [no inhibition]).

推荐实验方法(仅供参考)

激酶实验：

The inhibitory effect of T-00127_HEV1 at a concentration of 10μM on in vitro cellular protein kinase activities is assessed by kinase profiling with an ATP concentration near the Km for each kinase.Inhibitory effects of GW5074,AN-12-H5,and T00127_HEV1 at a concentration of 10μM on in vitro PI kinase activities are assessed by the SelectScreen kinase profiling service with an ATP concentration of 10μM.For T-00127_HEV1,the 50% inhibitory concentration (IC50) for in vitro PI4KB activity is also measured with an ATP concentration of 10μM.

细胞实验:

RD cells (1.0×104 cells per well in 100μL medium) in a 96-well plate are infected with PV1,EV71,or CVB3 at multiplicities of infection (MOI) of 10,1.0,and 0.1 at 37℃ for 1h in the absence of T-00127_HEV1. The cells are washed three times with 10% FCS-DMEM, followed by the addition of 100μL of 10% FCS-DMEM containing 10 or 0μM T-00127_HEV1.Cells are collected at 16h p.i.,and then viral RNA is extracted from the cells using a viral RNA purification kit.The number of copies of the viral genome is quantified using a real-time PCR system.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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