| 中文名称: | 苯烯莫德 | ||||
|---|---|---|---|---|---|
| 英文名称: | Tapinarof | ||||
| 别名: | 苯烯莫德 WBI-1001; Benvitimod; GSK2894512 | ||||
| CAS No: | 79338-84-4 | 分子式: | C17H18O2 | 分子量: | 254.32 |
| CAS No: | 79338-84-4 | ||||
| 分子式: | C17H18O2 | ||||
| 分子量: | 254.32 | ||||
基本信息
|
产品编号： |
T10995 |
||||
|
产品名称： |
Tapinarof |
||||
|
CAS: |
79338-84-4 |
储存条件 |
粉末 |
2-8℃ |
四年 |
|
分子式： |
溶于液体 |
-80℃(避光) |
6个月 |
||
|
分子量： |
254.32 |
-20℃(避光) |
1个月 |
||
|
化学名： |
|||||
|
Solubility (25°C)： |
|||||
|
体外:
|
DMSO |
51mg/mL (200.53mM) |
|||
|
Ethanol |
51mg/mL (200.53mM) |
||||
|
Water |
Insoluble |
||||
|
体内(现配现用): |
|
||||
|
＜1mg/ml表示微溶或不溶。 |
|||||
|
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
|||||
|
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
|||||
制备储备液
|
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
|
1mM |
3.9321mL |
19.6603mL |
39.3205mL |
|
5mM |
0.7864mL |
3.9321mL |
7.8641mL |
|
10mM |
0.3932mL |
1.9660mL |
3.9321mL |
|
50mM |
0.0786mL |
0.3932mL |
0.7864mL |
生物活性
|
产品描述 |
一种天然的芳烃受体(AhR)激动剂 |
|
|
靶点 |
AhR |
apoptosis |
|
0.16nM(EC50) |
5.2μM |
|
|
体外研究 |
Tapinarof activates the AhR pathway through direct binding.Tapinarof dose-dependently induces nuclear translocation of AhR in immortalized keratinocytes (HaCaT) (EC50=0.16nM). |
|
|
体内研究 |
Tapinarof acts through AhR to reduce inflammation in IMQ-treated mice.AhR-sufficient mice on a C57Bl/6 background exhibit a reduced clinical score after treatment with Tapinarof or 6-formylindolo(3,2-b)carbazole (FICZ).In contrast,AhR KO mice do not respond to the anti-inflammatory effects of Tapinarof.FICZ is used as a comparator in these studies and yields similar results,with dramatically reduced inflammatory responses in wild-type,but not AhR KO mice. |
|
推荐实验方法(仅供参考)
|
细胞实验: |
|
|
HaCaT cells (10,000 cells/well) are cultured in 96-well Greiner μCLEAR plates in 100μL DMEM with HEPES,Glutamax and 10% fetal bovine serum to confluence.Media is replaced with 100μL media containing 0.2% heat-inactivated,charcoalstripped fetal bovine serum and incubated overnight.Titrating concentrations of Tapinarof (10-8μM,10-6μM,10-4μM,0.01μM,and 1μM) are added for 30 minutes followed by washing and fixing in ice-cold methanol:acetone (50:50).Samples are blocked with 3% BSA for 1 hour,and then washed again in phosphate buffered saline with 0.1% Tween-20.Next,cells are stained with 50μL of 1:50 dilution anti-AhR antibody in 3% BSA,followed by 50μL secondary antibody (1:500 dilution chicken anti-rabbit AlexaFluor488 and 1:2,000 dilution Hoechst 33342) in 3% BSA/phosphate buffered saline.Images are acquired on InCell 2000 and/or Opera.Image analysis is performed using InCell Analyzer Workstation and/or Columbus. |
|
|
动物实验: |
|
|
Mice Female BALB/c mice (BALB/cByJRj) are used.Studies are performed using 100µL of Tapinarof (1%) or FICZ (0.01%,at the limit of solubility) in 60% ethanol:40% water. |
|
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
