| 中文名称: | SEA0400 | ||||
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| 英文名称: | SEA0400 | ||||
| 别名: | SEA0400 SEA0400 | ||||
| CAS No: | 223104-29-8 | 分子式: | C21H19F2NO3 | 分子量: | 371.38 |
| CAS No: | 223104-29-8 | ||||
| 分子式: | C21H19F2NO3 | ||||
| 分子量: | 371.38 | ||||
基本信息
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产品编号： |
S10965 |
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产品名称： |
SEA0400 |
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CAS: |
223104-29-8 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
371.38 |
-20℃ |
1个月 |
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化学名： |
2-[4-[(2,5-Difluorophenyl)methoxy]phenoxy]-5-ethoxybenzenamine |
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Solubility (25°C)： |
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体外:
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DMSO |
74mg/mL (199.25mM) |
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Ethanol |
74mg/mL (199.25mM) |
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Water |
Insoluble |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.6927mL |
13.4633mL |
26.9266mL |
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5mM |
0.5385mL |
2.6927mL |
5.3853mL |
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10mM |
0.2693mL |
1.3463mL |
2.6927mL |
生物活性
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产品描述 |
一种新颖的，具有选择性的Na+-Ca2+exchanger抑制剂，在培养的神经元、星形胶质细胞和小胶质细胞中，能够抑制Na+-依赖性Ca2+的吸收，IC50值为5-33nM。 |
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靶点 |
IC50:5-33nM (NCX) |
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体外研究 |
SEA0400 inhibits Na+-dependent 45Ca2+ uptake in cultured neurons,astrocytes,and microglia.IC50 values of SEA0400 are 33nM (neurons),5.0nM (astrocytes),and 8.3nM (microglia).SEA0400 prevents sodium nitroprusside (SNP) to increase ERK and p38 MAPK phosphorylation,and production of reactive oxygen species (ROS) in an extracellular Ca2+-dependent manner. |
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体内研究 |
SEA0400 (3mg/kg+3mg/kg/h for 2h,i.v.) attenuates the infarct volume in the cerebral cortex and striatum,does not affect the mean the regional cortical blood flow in anesthetized rats.SEA0400 protects against the dopaminergic neurotoxicity (determined by dopamine levels in the midbrain and striatum,tyrosine hydroxylase immunoreactivity in the substantia nigra and striatum,striatal dopamine release,and motor deficits) in MPTP-treated C57BL/6J mice. |
推荐实验方法(仅供参考)
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激酶实验： |
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Na+-Ca2+ exchange activity is determined by assaying Na+-dependent 45Ca2+uptake as reported previously.Briefly,the cells are preincubated in Hanks' balanced saline solution (HBSS) for 20 min,and the medium is switched to HBSS containing 45 Ca2+ and incubated for 5 min.To increase intracellular Na+concentration,1mM ouabain plus 20μM monensin (for astrocytes and microglia) and 10μM monensin (for neurons) are used.Monensin is added simultaneously with the isotope.Ouabain is added 5 min before monensin in astrocytes and microglia.SEA0400 and KB-R7943 are added 5 min before monensin and present during 45Ca2+ uptake reaction. |
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细胞实验: |
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Cells,plated in 96-well plastic tissue culture plates,are incubated at 37℃ for 30 min in normal or Ca2+-free HBSS containing 10μM H2DCF-DA and 0.25μg/mL Cremophor EL,and then rinsed twice with normal HBSS to remove excess dye.The cells are reperfused in normal HBSS for 1h,and the conversion of H2DCF-DA to its fluorescent product dichlorofluorescein by ROS,presumably H2O2 and hydroxyl radical,is determined with excitation at 485nm and emission at 535nm using a Wallac Multilabel counter.ROS production is expressed as a percentage of control cells.The linearity and sensitivity of ROS assay are confirmed using H2O2 prior to the experiment.SEA0400 at the indicated concentrations is added 10 min before Ca2+reperfusion and present until assay. |
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动物实验: |
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Male Sprague-Dawley rats,weighing 289 to 325g,are anesthetized with 1 to 2% halothane.A catheter is inserted into the femoral artery and connected to a pressure transducer to record blood pressure.Regional cortical blood flow is measured by a laser Doppler flowmeter,with probe placement at 2 mm posterior and 6 mm lateral to the bregma.SEA0400 or its vehicle with an equivalent volume is i.v.injected at 3mg/kg and then infused at 3mg/kg/h for 2h under normal conditions without MCA occlusion. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
