| 中文名称: | 吡罗达韦 | ||||
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| 英文名称: | Pirodavir | ||||
| 别名: | 吡罗达韦 R77975 | ||||
| CAS No: | 124436-59-5 | 分子式: | C21H27N3O3 | 分子量: | 369.46 |
| CAS No: | 124436-59-5 | ||||
| 分子式: | C21H27N3O3 | ||||
| 分子量: | 369.46 | ||||
基本信息
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产品编号：P10613 |
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产品名称：Pirodavir |
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CAS: |
124436-59-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
369.46 |
-20℃ |
一个月 |
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化学名： |
ethyl 4-(2-(1-(6-methylpyridazin-3-yl)piperidin-4-yl)ethoxy)benzoate |
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Solubility (25°C) |
体外 |
DMSO |
10 mg/mL (27.07mM; Need ultrasonic) |
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Ethanol |
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Water |
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体内 |
现配现用 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.7067mL |
13.5333mL |
27.0665mL |
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5mM |
0.5413mL |
2.7067mL |
5.4133mL |
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10mM |
0.2707mL |
1.3533mL |
2.7067mL |
生物活性
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产品描述 |
一种有效的小核糖核酸病毒抑制剂，高效作用于 A 和 B 型鼻病毒。 |
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靶点/IC50 |
Rhinovirus |
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体外研究 |
Pirodavir is a potent, broad-spectrum picornavirus inhibitor. Pirodavir inhibits 80 of the 100 human rhinovirus (HRV) strains tested at a concentration of 64ng/mL. In that same study, Pirodavir is also effective in inhibiting 16 enteroviruses, with a mean 80% inhibitory concentration (IC80) of 1,300ng/mL. Pirodavir inhibits enterovirus 71 replication with an IC50 of 5,420nM and an IC90 of >13,350nM. Pirodavir inhibits 56 rhinovirus laboratory strains and three of the clinical isolates tested. Pirodavir inhibits 59% of the serotypes and isolates with IC50s of <100nM. Pirodavir concentrations of 16 and 4μg/mL reduces cell growth by 66% (s.e.m. 0.75) and 28% (s.e.m. 0.25), respectively. Lower concentrations (1μg/mL) of Pirodavir are not inhibitory for cell growth. The 50% cytotoxic concentration of pirodavir for logarithmic cell growth at 37°C is 7μg/mL.Under the conditions of the antiviral assay (confluent HeLa cells at 33°C), the 50% cytotoxic concentration is >50μg/mL. |
推荐实验方法(仅供参考)
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细胞实验: |
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Cell Assay |
HeLa cells are seeded at a concentration of approximately 180,000 cells per dish in six-well plates containing 4mL of growth medium. Growth medium consist of Eagle's basal medium, supplemented with 5% fetal calf serum, 2% sodium bicarbonate,and 1% glutamine. After 24 h of incubation at 37°C in a humidified CO2 atmosphere, the growth medium is removed and replaced by the test solutions (fresh growth medium with or without various concentrations of the antiviral compounds). To assess the cytotoxicity of the antiviral compounds (e.g., Pirodavir), the number of living cells are determined present in triplicate cultures at the time of Pirodavir addition and every 24 h for 3 days. Following trypsinization, the number of viable cells for each drug concentration is counted in triplicate with a Coulter Counter. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
