PI4KIIIbeta-IN-10 - CAS:1881233-39-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	PI4KIIIbeta-IN-10
英文名称:	PI4KIIIbeta-IN-10
别名:	PI4KIIIbeta-IN-10;N-(5-(3-(N-(4-hydroxyphenyl)sulfamoyl)-4-methoxyphenyl)-4-methylthiazol-2-yl)pivalamide
CAS No:	1881233-39-1	分子式:	C₂₂H₂₅N₃O₅S₂	分子量:	475.58
CAS No:	1881233-39-1
分子式:	C₂₂H₂₅N₃O₅S₂
分子量:	475.58

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P10567 PI4KIIIbeta-IN-10 ≥98% (psaitong)

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PI4KIIIbeta-IN-10
CAS No：1881233-39-1

包装规格：

1mg 5mg 10mg 25mg in glass bottle

溶解性：

溶于DMSO（≥ 30 mg/mL)

产品描述：

基本信息

产品编号：	P10567
产品名称：	PI4KIIIbeta-IN-10
CAS:	1881233-39-1	储存条件	粉末	-20℃	四年

分子式：	C22H25N3O5S2		溶于液体	-80℃	6个月
分子量：	475.58			-20℃	1个月
化学名：	PI4KIIIbeta-IN-10;N-(5-(3-(N-(4-hydroxyphenyl)sulfamoyl)-4-methoxyphenyl)-4-methylthiazol-2-yl)pivalamide
Solubility (25°C)：
体外:	DMSO
	Ethanol
	Water
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.1027mL	10.5135mL	21.0270mL
5mM	0.4205mL	2.1027mL	4.2054mL
10mM	0.2103mL	1.0513mL	2.1027mL

生物活性

产品描述	一种有效的PI4KIIIβ抑制剂
靶点	PI4K
体外研究	PI4KIIIbeta-IN-10 (Compound 10) is a potent PI4KIIIβ inhibitor with very minor off-target inhibition of PI4KIIIβ related lipid kinases.PI4KIIIbeta-IN-10 shows weak inhibition of PI3KC2γ(IC50 ~1µM),PI3Kα(~10 µM),and PI4KIIIα(~3 µM),and <20% inhibition at concentrations up to 20µM for PI4K2α,PI4K2β,and PI3Kβ.

推荐实验方法(仅供参考)

激酶实验：

Lipid kinase assays are preformed using recombinant enzyme,phosphoinositides and γ32P-ATP in a membrane capture assay.Each inhibitor (e.g.,PI4KIIIbeta-IN-10) is diluted into 10% DMSO and kinase assay buffer.Upon completion of the reaction,4µL is spotted onto 0.2µm nitrocellulose.The membrane is dried for 5 minutes under a heat lamp followed by 1×30 second wash and 6×5 min washes in 1M NaCl/1% Phosphoric Acid.The membrane is dried for 20 minutes under a heat lamp followed by overnight exposure to a phosphor screen and phosphorimaging followed on a Typhoon 9500.Intensities are quantified using SPOT. Specifications for each enzyme follow.L-α-Phosphatidylinositol and DOPS:DOPC lipids are sonicated in water to generate 1mg/mL PI:DOPS:DOPC.Reaction is set-up as follows 1) kinase assay buffer,PI:DOPS:DOPC,BSA and PI4KIIIβ,are combined in a total volume of 10µL (2.5x solution);2) 5µL of inhibitor solution is added (5x solution) and incubated with enzyme mixture for 15 minutes;3) 10µL cold ATP and γ32P-ATP are added (2.5x solution) to initiate the reaction which ran for 30 minutes.Final conditions are as follows:20mM Bis-Tris Propane pH 7.5,10mM MgCl2,0.075mM Triton X-100,0.5mM EGTA,1mM DTT,100µM PI,500ng/µL BSA,2.5nM PI4KIIIβ,2% DMSO,10µM ATP and 1 uCi γ32P-ATP.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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