Nα-甲苯磺酰基-L-赖氨酸氯甲基酮盐酸盐 - CAS:4272-74-6 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Nα-甲苯磺酰基-L-赖氨酸氯甲基酮盐酸盐
英文名称:	Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride
别名:	Nα-甲苯磺酰基-L-赖氨酸氯甲基酮盐酸盐 (S)-N-(7-Amino-1-chloro-2-oxoheptan-3-yl)-4-methylbenzenesulfonamide hydrochloride;TLCK
CAS No:	4272-74-6	分子式:	C₁₄H₂₁ClN₂O₃S^.HCl	分子量:	369.31
CAS No:	4272-74-6
分子式:	C₁₄H₂₁ClN₂O₃S^.HCl
分子量:	369.31

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

N70096 Nα-甲苯磺酰基-L-赖氨酸氯甲基酮盐酸盐 ≥98% (psaitong)

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包装规格：

25mg 50mg 100mg in glass bottle

溶解性：

溶于水(50mg/mL)

产品描述：

基本信息

产品编号：	N70096
产品名称：	Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride
CAS:	4272-74-6	储存条件	粉末	-20℃	四年

分子式：	C14H21ClN2O3S.HCl		溶于液体	-80℃	六个月
分子量	369.31			-20℃	一个月
化学名：	(S)-N-(7-Amino-1-chloro-2-oxoheptan-3-yl)-4-methylbenzenesulfonamide hydrochloride
Solubility (25°C)：
体外:	DMSO	250mg/mL (676.94mM; Need ultrasonic)
	Ethanol
	Water	100mg/mL (270.78mM; Need ultrasonic)
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.08mg/mL (5.63mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (5.63mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08mg/mL (5.63mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (5.63mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (5.63mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (5.63mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.7078mL	13.5388mL	27.0775mL
5mM	0.5416mL	2.7078mL	5.4155mL
10mM	0.2708mL	1.3539mL	2.7078mL

生物活性

产品描述

阻断脂多糖（LPS）或细胞因子诱导的核因子κB（NF-κB）激活，进而阻断诱导型一氧化氮合酶（iNOS）和环氧合酶-2（COX-2）转录的诱导。

体外研究

N-alpha-Tosyl-L-lysine chloromethyl ketone exhibits an inhibitory effect on IFN-γ activities. The effect of TLCK is studied on the IFN-γ sensitization of HeLa cells towards cell death mediated by anti-Fas. Lower concentration of anti-Fas (10 ng/mL) are used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK and IFN-γ. TLCK by itself up to 50μM concentration exhibits a small decrease in cell viability. Beyond 50μM, a dose dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. Addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced upon addition of higher concentrations of TLCK, 25μM and more

推荐实验方法(仅供参考)

Cell Assay

HeLa cell line (human cervical cancer cells) is cultured in DMEM supplemented with 10% fetal bovine serum (FBS), Lglutamine (300mg/L), penicillin (100 U/ml) and streptomycin(100μg/ml) at 37°C in 5% CO2. HT-29 cell line (human colorectal adenocarcinoma) is cultured in RPMI supplemented with 10% fetal bovine serum (FBS), L-glutamine (300mg/L), penicillin (100 U/mL) and streptomycin (100μg/mL) at 37°C in 5% CO2. The cells are split every second day to keep the cell growth in logarithmic phase. The cells are routinely tested for mycoplasma. The cells are treated with different concentrations of TPCK or TLCK (5, 10, 25, 50, 100, 150, and 200μM) for 30 min and/or with different concentrations of IFN-γ for 2 h followed by treatment with different concentrations of anti-Fas for 2 or 4 days for each specific experiment. The control cells are treated with the respective vehicle only. Cell viability analysis of HeLa and HT-29 cells is assessed by their XTT reduction activity. 100μL of 2×10⁴ cells/mL is incubated with treatments at the indicated time. At the end of the incubation period, 25μL of 1mg/mL XTT solution (containing 0.2mM phenazine methosulphate (PMS) is added and the cells are incubated for an additional 1 h. The OD values are measured using an ELISA reader at 450nm with a reference wavelength of 650nm

保存条件：

-20°C

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果