| 中文名称: | MS-444 | ||||
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| 英文名称: | MS-444 | ||||
| 别名: | BE-34776 | ||||
| CAS No: | 150045-18-4 | 分子式: | C13H10O4 | 分子量: | 230.22 |
| CAS No: | 150045-18-4 | ||||
| 分子式: | C13H10O4 | ||||
| 分子量: | 230.22 | ||||
基本信息
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产品编号：M10860 |
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产品名称：MS-444 |
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CAS: |
150045-18-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃（避光，储存于氮） |
六个月 |
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分子量 |
371.45 |
-20℃（避光，储存于氮） |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
4.3437mL |
21.7184mL |
43.4367mL |
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5mM |
0.8687mL |
4.3437mL |
8.6873mL |
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10mM |
0.4344mL |
2.1718mL |
4.3437mL |
生物活性
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产品描述 |
是平滑肌肌球蛋白轻链激酶 (MLCK) 的抑制剂，IC50 值为 10μM。 |
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靶点/IC50 |
10μM (myosin) |
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体外研究 |
MS-444 is a small molecule RNA-binding protein HuR (ELAVL1) inhibitor. Colorectal cancer (CRC) cells that display HuR overexpression are treated with MS-444 (1-100μM) for 48 hr with IC50s of 10.98±1.76μM,12.84±2.10μM,5.60±0.90μM,14.21±2.11μM,and 10.98±1.24μM for HCT116, HCA-7, RKO, HT-29, and SW480 cells,respectively. Growth inhibition is observed in all CRC lines with IC50 values ranging from 5.60μM to 14.21μM with observable effects seen at 10μM MS-444.Contrasting effects are observed using non-transformed small intestinal (RIE-1 (IC50=40.70±3.53μM)) and colonic (YAMC (IC50=28.16±3.23μM)) epithelial cells. Both cell types display properties of normal intestinal epithelial cells and are proficient in 3′UTR AU-rich elements (ARE)-mRNA decay. Both non-transformed cell lines are ~3- to 4-fold less responsive to MS-444-mediated growth inhibition, with IC50 values of 40.70μM and 28.16μM (P<0.05). |
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体内研究 |
To test the effects of MS-444 on CRC cell growth in vivo, mice bearing HCT116 cell xenografts receive IP injections of MS-444 (25mg/kg bw) or vehicle every 48hr. Over the experiment course, mice do not display any adverse effects and maintained similar weights. Anti-tumor effects of MS-444 are observed with approximately 1.7-fold reduction in tumor size. Mice treated with MS-444 show a marked 2- to 3-fold decrease in microvessel density (MVD), indicating the anti angiogenic potential of MS-444. |
推荐实验方法(仅供参考)
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细胞实验: |
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Human colorectal cancer cell lines RKO, HCA-7, HCT116, HT-29,SW480 and the non-transformed intestinal epithelial cell lines RIE-1, YAMC are treated with varying concentrations of MS-444 (1-100μM) for 48 hr. Cell survival is measured by MTT assay after incubation of cells for 48 hr with MS-444. Relative cell survival is calculated as percentage normalized to DMSO vehicle-treated cells and plotted to determine IC50. |
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动物实验: |
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Mice Athymic nude (Nu/Nu) mice are used. HCT116 (2×106 cells) and HCA-7 (2.5×106) cells resuspended in PBS are injected into the dorsal subcutaneous tissue. Mice (n=5 per group) receive intraperitoneal (IP) injections of MS-444 (25mg/kg) dissolved in PBS/5% N-Methyl Pyrrolidine (NMP) or vehicle control every 48 hr. Tumor growth is assayed. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
