L-犬尿氨酸 - CAS:2922-83-0 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	L-犬尿氨酸
英文名称:	L-Kynurenine
别名:	β-氨茴酰-L-丙氨酸 L-Kynurenine
CAS No:	2922-83-0	分子式:	C₁₀H₁₂N₂O₃	分子量:	208.21
CAS No:	2922-83-0
分子式:	C₁₀H₁₂N₂O₃
分子量:	208.21
MDL:	MFCD00069912	EINEC:	4425
EINEC:	4425

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K10065 L-犬尿氨酸 ≥98% (psaitong)

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包装规格：

25mg 100mg 500mg in glass bottle

产品描述：

基本信息

产品编号：	K10065
产品名称：	L-Kynurenine
CAS:	2922-83-0	储存条件	粉末	室温	四年

分子式：	C10H12N2O3		溶于液体	-80℃	两年
分子量	208.21			-20℃	一个月
化学名：	(2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid
Solubility (25°C)：
体外:	DMSO	42mg/mL (201.71mM)
	Ethanol	Insoluble
	Water	3mg/mL (14.4mM)
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (9.99mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (9.99mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
	2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 0.83mg/mL (3.99mM); Clear solution
	此⽅案可获得 ≥ 0.83mg/mL (3.99mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 8.3mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	3.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 0.83mg/mL (3.99mM); Clear solution
	此⽅案可获得 ≥ 0.83mg/mL (3.99mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 8.3mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	4.8028mL	24.0142mL	48.0284mL
5mM	0.9606mL	4.8028mL	9.6057mL
10mM	0.4803mL	2.4014mL	4.8028mL
50mM	0.0961mL	0.4803mL	0.9606mL

生物活性

产品描述	L-犬尿氨酸是L-色氨酸降解的关键中介物，通过犬尿氨酸途径形成烟酰胺腺嘌呤二核苷酸(NAD+)。可用作吲哚胺-2,3-双加氧酶分析标准；从培养细胞和培养基中提取和定量犬尿氨酸的标准。
靶点	Human Endogenous Metabolite
体外研究	Kynurenine and its further breakdown products carry out diverse biological functions, including dilating blood vessels during inflammation and regulating the immune response. Some cancers increase kynurenine production, which increases tumor growth. L-kynurenine (Kyn) is an aryl hydrocarbon receptor (AHR) agonist that activates AHR-directed, naive T cell polarization to the anti-inflammatory Treg phenotype. Kynurenine activates AHR signaling at physiological concentrations in H1L7.5c3 cells and acts as an AHR agonist after a 24-hr exposure by inducing the AHR-regulated luciferase gene in H1L7.5c3 mouse hepatocyte cells
体内研究	Kynurenine dilates arteries from rats as well as humans via Kv7 channels in the vascular smooth muscle. In rats, this tryptophan metabolite causes hypotension, which is partly counteracted by Kv7 channel inhibition. L-kynurenine administered 1 h before the hypoxia-ischemia shows a dose-dependent significant neuroprotective effect, with complete protection at a dose of 300mg/kg. The induction of c-fos immunoreactivity in cerebral cortex is also blocked by this dose of L-kynurenine

推荐实验方法(仅供参考)

Cell Assay

Luciferase assays are carried out using the H1L7.5c3 cells. At the indicated times (0.5, 2, 4, 6, 12, 18, 24 h) and concentrations (0.1, 1, 10, 100μM) of exposures to Kynurenine, cells are removed from incubation and allowed to equilibrate to room temperature for 15min. After equilibration, the medium is removed and the cells are washed twice with at room temperature with DPBS. The cells are lysed with 20μL/well 1× Passive Lysis Buffer and shaken for 20min at room temperature. Luciferase activity is recorded using an Luminometer Microplate Reader

Animal Administration

Rats

The effects of increasing doses of L-kynurenine with or without probenecid on concentrations of kynurenic acid in cerebral cortex are examined in 7-day-old rats. Six animals are examined in each group. Animals are treated with L-kynurenine at doses of 100, 200, 300, and 400mg/kg or kynurenine, 200mg/kg with probenecid, 50mg/kg. Animals are killed at 1 h, the brains promptly removed, and the cerebral cortex is dissected and placed in 0.5mL of chilled 0. 1 M HCl. Kynurenic acid measurements are made by high-performance liquid chromatography with fluorescence detection. Protein measurements are made using a fluorometric assay

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