ISCK03 - CAS:945526-43-2 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	ISCK03
英文名称:	ISCK03
别名:	Benzenesulfonamide,4-(1,1-dimethylethyl)-N-[4-(1H-imidazol-1-yl)phenyl] [4-t-Butylphenyl]-N-(4-imidazol-1-yl phenyl)sulfonamide
CAS No:	945526-43-2	分子式:	C₁₉H₂₁N₃O₂S	分子量:	355.45
CAS No:	945526-43-2
分子式:	C₁₉H₂₁N₃O₂S
分子量:	355.45
MDL:	MFCD12031591

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

I10017 ISCK03 ≥98%(HPLC) (psaitong)

相关产品

包装规格：

5mg 25mg 50mg 100mg in glass bottle

溶解性：

溶于DMSO( ≥20 mg/mL )

产品描述：

基本信息

产品编号：I10017
产品名称：ISCK03
CAS:	945526-43-2	储存条件	粉末	-20℃	四年

分子式：	C19H21N3O2S		溶于液体	-80℃	六个月
分子量	355.45			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	71mg/mL (199.74mM)
		Ethanol	3mg/mL (8.44mM)
		Water	Insoluble
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.8133mL	14.0667mL	28.1334mL
5mM	0.5627mL	2.8133mL	5.6267mL
10mM	0.2813mL	1.4067mL	2.8133mL
50mM	0.0563mL	0.2813mL	0.5627mL

生物活性

产品描述	一种c-kit活性抑制剂。
靶点/IC₅₀	SCF	c-Kit
体外研究	Pretreatment of 501mel cells with ISCK03 inhibits SCF-induced c-kit phosphorylation dose dependently. ISCK03 also inhibits p44/42 ERK mitogen-activated protein kinase (MAPK) phosphorylation, which is known to be involved in SCF/c-kit downstream signaling. However ISCK03 does not inhibit hepatocyte growth factor (HGF)-induced phosphorylation of p44/42 ERK proteins. ISCK03, a tyrosine kinase inhibitor specific to KIT, prevents survival of CCDC26-KD cells under low-serum conditions. All treated cells exhibits sensitivity to ISCK03 in a dose-dependent manner. After ISCK03 treatment, the survival of KD cells is suppressed to the same level as that of non-KD cells. Conversely, ISCK03 treatment has limited effects on the growth of control K562 and KD clone 3–4 cells under high-serum concentration conditions.
体内研究	Oral administration of ISCK03 induces the dose-dependent depigmentation of newly regrown hair, and this is reversed with cessation of ISCK03 treatment. The topical application of ISCK03 promotes the depigmentation of UV-induced hyperpigmented spots. Fontana-Masson staining analysis shows epidermal melanin is diminished in spots treated with ISCK03.

推荐实验方法(仅供参考)

激酶实验：

ATP is dispensed into 384-well plates, chemical compounds (ISCK03: 2.5, 5, 10, 100μM) are added by replicative plate, and recombinant human c-kit protein is added for the kinase reaction.Following a 45-min incubation at 37℃, the development reaction is carried out for 40min at room temperature. After the reaction is stopped, the coumrain and fluorescein fluorescence-emission signals are detected.

细胞实验:
To determine any cytotoxic effects of ISCK03 on 501mel cells, MTT assays are performed with various doses of ISCK03 (1, 5,10μM). 501mel cells are cultured with SCF alone (50ng/mL) or SCF with ISCK03 for 48h.

动物实验:

Mice: To induce the hair cycle, depilation of skin on the back of the female C57BL/6 mice is performed. Briefly, the hair is removed from anesthetized mice. The rat antimouse c-kit-neutralizing monoclonal antibody ACK2 is administered intraperitoneally (50mg) every day.ISCK03 is administered orally once a day. On days 21–28, animals are sacrificed or analyzed for repigmentation of the newly regrown hair shaft. Skin is harvested and fixed in paraffin or frozen for immunohistochemical analyses.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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