H100 - CAS:643727-55-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	H100
英文名称:	H100
别名:	H100 H100
CAS No:	643727-55-3	分子式:	C₁₈H₁₆N₂O₆S	分子量:	388.39
CAS No:	643727-55-3
分子式:	C₁₈H₁₆N₂O₆S
分子量:	388.39

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H10479 H100 (psaitong)

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包装规格：

1mg in glass bottle

产品描述：

基本信息

产品编号：	H10479
产品名称：	H100
CAS:	643727-55-3	储存条件	粉末	-20℃	四年
分子式：	C18H16N2O6S		溶于液体	-80℃	一年
分子量：	388.39
化学名：
Solubility (25°C)：
体外:	DMSO
	Ethanol
	Water
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

生物活性

产品描述

一种氯离子运输(Cl- transport)抑制剂，可以部分抑制氯离子载体NaK2Cl cotransporter和Band 3 anion exchanger，但是对KCl cotransporter无作用。

靶点

Cl- transport

体外研究

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE),but no effect against KCl cotransporter (KCC),in human erythrocytes.H100 (0.1mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes.

推荐实验方法(仅供参考)

细胞实验:

35SO4 2- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE).Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37℃ for 30 min.The cell suspension is then centrifuged (3,000g,5 min) and the supernatant removed.The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO4 2-.To load the erythrocytes with radiolabel,the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO4 2- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4,300±5 mOsm) and placed in a microcentrifuge tube.10μCi of 35SO4 2- is then added and the suspension incubated at 37℃ for 1h.At the end of this period,the cells are then washed four times by centrifugation (10,000g,10s) in ice-cold SO4 2- MBS.All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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