小檗碱 - CAS:2086-83-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	小檗碱促销
英文名称:	Berberine
别名:	黄连素 Berberine
CAS No:	2086-83-1	分子式:	C₂₀H₁₈NO₄+	分子量:	336.37
CAS No:	2086-83-1
分子式:	C₂₀H₁₈NO₄+
分子量:	336.37
MDL:	MFCD01175817	EINEC:	218-229-1
EINEC:	218-229-1

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B10114 小檗碱 ≥97% (psaitong)

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包装规格：

25mg 100mg 1g in glass bottle

产品描述：

基本信息

产品编号：	B10114
产品名称：	Berberine
CAS:	2086-83-1	储存条件	粉末	2-8℃	四年

分子式：	C20H18NO4+		溶于液体	-80℃	两年
分子量	336.37
化学名：	9,10-dimethoxy-5,6-dihydro[1,3]dioxolo[4,5-g]isoquino[3,2-a]isoquinolin-7-ium
Solubility (25°C)：
体外:	DMSO	22.5mg/mL(66.89mM)
	Ethanol	5mg/mL(14.86mM)
	Water	1mg/mL(2.97mM)
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.9730mL	14.8650mL	29.7301mL
5mM	0.5946mL	2.9730mL	5.9460mL
10mM	0.2973mL	1.4865mL	2.9730mL
50mM	0.0595mL	0.2973mL	0.5946mL

生物活性

产品描述	Berberine (Natural Yellow 18) 是从中草药⻩连中分离出来的⼀种⽣物碱，常⽤作抗⽣素。Berberine (Natural Yellow 18) 诱导活性氧 (ROS) ⽣成并抑制 DNA 拓扑异构酶 (topoisomerase)。Berberine (Natural Yellow 18) 具有抗肿瘤特性。
靶点	Human Endogenous Metabolite
体外研究	Berberine (1.25-160μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29. Berberine (1.25-160μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth. LoVo cells are exposure to Berberine (10-80μM) for 24 h. Cell cycle analysis of 40μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase. Berberine (10-80μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0μM Cell Proliferation Assay
	Cell Line:	Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
	Concentration:	1.25, 2.5, 5, 10, 20, 40, 80, and 160μM
	Incubation Time:	72 hours
	Result:	Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1μM (LoVo) to 98.6±2.9μM (HCT116).
	Cell Proliferation Assay
	Cell Line:	Colorectal carcinoma cell lines LoVo
	Concentration:	1.25, 2.5, 5, 10, 20, 40, 80, and 160μM
	Incubation Time:	24, 48, 72 hours
	Result:	Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0μM induced 71.1±1.9 % growth inhibitions in LoVo cells.
	Cell Cycle Analysis
	Cell Line:	LoVo cells
	Concentration:	0, 10, 20, 40, or 80μM
	Incubation Time:	24 hours
	Result:	Exposure to 40.0μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.
	Western Blot Analysis
	Cell Line:	LoVo cells
	Concentration:	10, 20, 40, or 80μM
	Incubation Time:	24 hours
	Result:	Suppressed cyclin B1, cdc2 and cdc25c protein expression.
体内研究	Berberine (10, 30, or 50mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice
	Animal Model:	5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts
	Dosage:	10, 30, or 50mg/kg/day
	Administration:	Gastrointestinal gavage; for 10 consecutive days
	Result:	Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50mg/kg/day.