| 中文名称: | 3-TYP 促销 | ||||
|---|---|---|---|---|---|
| 英文名称: | 3-TYP | ||||
| 别名: | 3-(1H-1,2,3-triazol-4-yl) pyridine | ||||
| CAS No: | 120241-79-4 | 分子式: | C7H6N4 | 分子量: | 146.15 |
| CAS No: | 120241-79-4 | ||||
| 分子式: | C7H6N4 | ||||
| 分子量: | 146.15 | ||||
| MDL: | MFCD25956467 | ||||
基本信息
|
产品编号：T10037 |
|||||
|
产品名称：3-TYP |
|||||
|
CAS: |
120241-79-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
|
|
|
||||
|
分子式： |
溶于液体 |
-80℃ |
两年 |
||
|
分子量 |
146.15 |
-20℃ |
一个月 |
||
|
化学名： |
|||||
|
Solubility (25°C) |
体外 |
DMSO |
29mg/mL (198.42mM) |
||
|
Ethanol |
29mg/mL (198.42mM) |
||||
|
Water |
1.25mg/mL warmed with 50ºC water bath (8.55mM) |
||||
|
体内 |
现配现用 |
|
|||
|
＜1mg/ml表示微溶或不溶。 |
|||||
|
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
|||||
|
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
|||||
制备储备液
|
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
|
1mM |
6.8423mL |
34.2114mL |
68.4229mL |
|
5mM |
1.3685mL |
6.8423mL |
13.6846mL |
|
10mM |
0.6842mL |
3.4211mL |
6.8423mL |
|
50mM |
0.1368mL |
0.6842mL |
1.3685mL |
生物活性
|
产品描述 |
一种选择性的SIRT3抑制剂，IC50:16nM。有比对SIRT1和SIRT2更强的选择性，对其IC50值为88nM 和 92nM。 |
||
|
靶点/IC50 |
SIRT3 |
SIRT1 |
SIRT2 |
|
16nM |
88nM |
92nM |
|
|
体外研究 |
3-TYP inhibits melatonin-enhanced SIRT3 activity but does not affect SIRT3 protein expression. 3-TYP pretreatment reverses the protective effects of melatonin on cadmium (Cd)-induced mitochondrial-derived O2•− production and autophagic cell death. 3-TYP significantly attenuates melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity in HepG2 cells exposed to Cd. |
||
|
体内研究 |
3-TYP (50mg/kg, i.p.) does not significantly influence the LVEF, LVFS, infarct size, serum LDH levels, apoptosis, and oxidative stress compared with those of the Sham group. Moreover, 3-TYP has little effect on gp91phox, Nrf2, NQO 1, Bax, Bcl-2,Caspase-3, and cleaved Caspase-3 expression levels, compared with the Sham group. 3-TYP significantly decreases SIRT3 activity and increases the acetylation of SOD2 compared with that in the control group, without influencing SIRT3 expression. 3-TYP attenuates the cardioprotective effects of melatonin by decreasing the LVEF and LVFS after 24 hour of reperfusion. 3-TYP also increases the infarct size, serum LDH levels, and apoptotic ratio compared with those in the IR+Mel group. |
||
推荐实验方法(仅供参考)
|
细胞实验： |
|
|
Cell Assay |
Cell viability is analyzed using Cell Counting Kit-8. Briefly, 1×104 cells are inoculated into 96-well plates. After being treated,90μL of medium and 10μL of CCK-8 solution are added to each well. The cells are then incubated at 37°C for 2 h. After incubation, the absorption at 450nm is measured using an Infinite™ M200 Microplate Reader. The results are expressed as a percentage of the control. The cell death is also evaluated using the trypan blue assay. HepG2 cells are plated in the 6-well plates (5×105 cells per well) and incubated for 24 h. After being treated with Cd or melatonin, the cells are detached with 300μL trypsin-EDTA solution. The mixture of detached cells is centrifugated at 300 g for 5 min. Then, the residue is combined with 800μL trypan blue solution and dispersed. After 3 min staining, cells are counted using an automated cell counter. The dead cells are stained with the blue color. Cell mortality (%) is expressed as percentage of the dead cell number/the total cell number. |
|
动物实验: |
|
|
Animal Administration |
In brief, male C57BL/6 mice are anesthetized with 2% isoflurane, and myocardial ischemia is produced by temporarily exteriorizing the heart via a left thoracic incision and placing a 6-0 silk suture slipknot around the left anterior descending coronary artery. After 30 minutes of myocardial ischemia, the slipknot is released, and the myocardium is reperfused for 3 hour (for western blot analysis and oxidative stress measurement) or 24 hour (for cardiac function, apoptotic index and infarct size determination). Sham-operated mice undergo the same surgical procedures except the suture placed under the left coronary artery is not tied. Ten minutes before reperfusion, mice are randomized to receive either vehicle (1% ethanol) or melatonin (20mg/kg) by intraperitoneal injection. C57BL/6 mice are randomly divided into the following groups: (i) Sham group: mice underwent the sham operation and are treated with vehicle (1% ethanol); (ii) Mel group: mice are treated with melatonin (20mg/kg via intraperitoneal injection); (iii) IR+V group: mice underwent the MI/R operation and are treated with vehicle (1% ethanol); (iv) IR+Mel group: mice underwent the MI/R operation and are treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); (v) IR+Mel+3-TYP group: mice are pretreated with 3-TYP (3-TYP is intraperitoneally injected at a dose of 50mg/kg every 2 days for a total of three doses prior to the MI/R surgery), subjected to the MI/R operation, and treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); and (vi) IR+3-TYP group: mice are pretreated with 3-TYP and then subjected to the MI/R operation. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
