SC66 - CAS:871361-88-5 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	SC66 促销
英文名称:	SC66
别名:	(2E,6E)-2,6-Bis(4-pyridinylmethylene)-cyclohexanone
CAS No:	871361-88-5	分子式:	C₁₈H₁₆N₂O	分子量:	276.33
CAS No:	871361-88-5
分子式:	C₁₈H₁₆N₂O
分子量:	276.33

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S10041 SC66 ≥98% (HPLC) (psaitong)

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包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO(≥5 mg/mL 加热）

产品描述：

基本信息

产品编号：	S10041
产品名称：	SC66
CAS:	871361-88-5	储存条件	粉末	-20℃	四年

分子式：	C18H16N2O		溶于液体	-80℃	6个月
分子量：	276.33			-20℃	1个月
化学名：	(2E,6E)-2,6-Bis(4-pyridinylmethylene)-cyclohexanone
Solubility (25°C)：
体外:	DMSO	55mg/mL (199.03mM)
	Ethanol
	Water
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(9.05mM);Clear solution
	此⽅案可获得 ≥ 2.5 mg/mL (9.05mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	2.请依序添加每种溶剂：10% DMSO→90%(20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(9.05mM);Clear solution
	此⽅案可获得 ≥ 2.5 mg/mL (9.05mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(9.05mM);Clear solution
	此⽅案可获得 ≥ 2.5 mg/mL (9.05mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.6189mL	18.0943mL	36.1886mL
5mM	0.7238mL	3.6189mL	7.2377mL
10mM	0.3619mL	1.8094mL	3.6189mL

生物活性

产品描述	一种Akt的变构抑制剂。
靶点	Akt
体外研究	SC66 inhibits cell viability and colony forming capacity of HCC cells with IC50s of 0.77，0.47，0.92，0.75 and 2.85μg/mL at 72 hours for HepG2,Hep3B,PLC/PRF/5，HA22T/VGH and Huh7 cells.HepG2,HA22T/VGH and PLC/PRF/5 cells have similar IC50s of approximately 0.85 and 0.75μg/mL at 48 and 72 hours,respectively.To determine whether the decrease in cell viability is related to apoptosis induction,TUNEL assays are performed in Hep3B and Huh7 cells treated with 1,2 and 4μg/mL of SC66 for 24 hours.In Hep3B cells the number of TUNEL-positive cells increased with increasing concentrations of SC66,whereas in Huh7 cells very few light brown-colored cells are observed only after treatment with 4μg/mL SC66.
体内研究	To demonstrate the effectiveness in vivo of SC66 on HCC,a mouse xenograft tumor model of Hep3B cells is used.When tumors became palpable,at a size of about 150 mm3,mice are randomized into three groups of 6 animals each.The treated group receive SC66 at 15 and 25mg/kg twice a week via i.p.injection,while the untreated group receive the vehicle alone.Treatment with 25mg/Kg SC66 significantly reduces tumor volume to 37% on day 17 when compared with tumors of the untreated group.

推荐实验方法(仅供参考)

细胞实验:

Cells (5×103/well) are distributed into each well of 96-well microtiter plates and then incubated overnight.At time 0,the medium is replaced with fresh complete medium and different doses of SC66 are added.Cells are cultured for 24,48 and 72 hours.At the end of treatment,MTS assays are performed using the CellTiter Aqueous OneSolution kit.Cell viability is expressed as a percentage of the absorbance measured in the control cells.Values are expressed as means±SD of three separate experiments,each performed in triplicate.

动物实验:

Mice

Male nude athymic mice (Fox1 nu/nu) aged 4 weeks are used.Suspensions of 1×107 Hep3B cells in 0.2mL of PBS are inoculated into the right flank of the animal.When tumors became palpable (around 150 mm3),the mice are randomly divided into three groups of 6 animals each,with the various tumor volumes equally distributed among the three groups.Two groups of mice are treated twice a week with 15 and 25mg/kg SC66 suspended in DMSO,further diluted in a solution of 25% ethanol and administered via i.p.injection.The control group receive the vehicle alone.Tumor volumes are determined twice a week using calipers. Primary tumor volumes are calculated.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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