雷西莫特 - CAS:144875-48-9 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	雷西莫特促销
英文名称:	Resiquimod
别名:	4-Amino-2-(ethoxymethyl)-alpha R848
CAS No:	144875-48-9	分子式:	C₁₇H₂₂N₄O₂	分子量:	314.38
CAS No:	144875-48-9
分子式:	C₁₇H₂₂N₄O₂
分子量:	314.38
MDL:	MFCD00937759

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R10022 雷西莫特 ≥98%(HPLC) (psaitong)

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包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO( 10mg/mL 加热至60 °C）

产品描述：

基本信息

产品编号：	R10022
产品名称：	Resiquimod
CAS:	144875-48-9	储存条件	粉末	-20℃	四年

分子式：	C17H22N4O2		溶于液体	-80℃	6个月
分子量：	314.38			-20℃	1个月
化学名：	4-Amino-2-(ethoxymethyl)-alpha R848
Solubility (25°C)：
体外:	DMSO	62mg/mL (197.21mM)
	Ethanol	27mg/mL warmed with 50ºC water bath (85.88mM)
	Water	Insoluble
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.1809mL	15.9043mL	31.8086mL
5mM	0.6362mL	3.1809mL	6.3617mL
10mM	0.3181mL	1.5904mL	3.1809mL
50mM	0.0636mL	0.3181mL	0.6362mL

生物活性

产品描述	一种作用于TLR7和TLR8免疫反应调节剂，EC50分别为0.26μm和6.4μm。
靶点	TLR7	TLR8
体外研究	Resiquimod (R-848) induces both hapten- and allergen-specific circulating T cells,including TH2 effectors,to produce IFN-γ and even to lose the ability to produce IL-4.Resiquimod (R848) enhances PBL proliferation in a dose-dependent manner,and increases the number of BrdU-positive cells in BrdU incorporation assay.Cells treated with R848 exhibits significantly increased (3.5-fold) luciferase (a reporter of NF-κB activity) activity.
体内研究	Resiquimod (R-848) (50μg/bird,i.m.route) significantly up-regulates the expression of IFN-α,IFN-β,IFN-γ,IL-1β,IL-4,iNOS and MHC-II genes in SPF chicken.

推荐实验方法(仅供参考)

激酶实验：

For luciferase assay,FG-9307 cells are transfected with the firefly NF-κB-specific luciferase reporter vector pNFκB-Met-Luc2.Transfection efficiency is monitored by co-transfection with the pSEAP2 control vector,which constitutively expresses the human secreted enhanced alkaline phosphatase (SEAP).Then the cells are treated with Resiquimod (R848,1µg/mL),CQ (10µM),CQ plus R848 or PBS and incubated at 22℃ for 24h.The culture medium of the transfectants is then analyzed for luciferase activity and SEAP activity using Luciferase Assay Kit and the Great EscAPe™ SEAP Chemiluminescence Detection Kit,respectively.The assay is performed three times.

细胞实验:

For inhibition of lysosomal acidification,cells are incubated with 10µM CQ for 1h before Resiquimod (R848) treatment.After treatment,20µL of 5mg/mL MTT is added to the plate.The plate is incubated at 22℃ for 4h,and 200µL dimethyl sulfoxide is added to the plate to dissolve the reduced formazan.The plate is then read at 490nm with a microplate reader.To determine the effect of Myd88 inhibition on R848-induced cell proliferation,the Myd88 inhibitor Pepinh-MYD andthe control peptide Pepinh-Control are added to PBL at the concentration of 50µM,and the plate is incubated at 22℃ for 6h.After incubation,the cells are treated with R848 and subjected to MTT assay as above.To determine the effect of NF-κB inactivation on R848-induced cell proliferation,BAY-11-7082,an irreversible inhibitor of IκB-α phosphorylation,is added to the cells at the concentration of 1µM,and the plate is incubated at 22℃ for 1h.After incubation,the cells are treated with R848 and subjected to MTT assay as earlier.All experiments are performed three times.

动物实验:

A total of 40 SPF chickens of two-week old are allotted to one of the following four experimental groups (n=10/group):Group A:PBS control;Group B:inactivated NDV vaccine;Group C:commercial oil adjuvanted inactivated NDV vaccine prepared from lentogenic strain and Group D:combination of inactivated NDV vaccine and R-848 (50μg/bird).Vaccine or PBS is administered by intramuscular route in the thigh muscle.A booster dose is given 14-day post immunization (d.p.i).Two weeks post-booster,experimental SPF birds are challenged with velogenic strain of NDV (105 ELD50 per bird) intramuscularly.Clinical signs and mortality are observed daily till 14 day post-challenge (d.p.c).Cloacal swabs (n=6/group) are collected from the birds on day 0,4,7 and 14 post-challenge and inoculated into 10-day old embryonated chicken eggs (n=3 eggs/sample) through intra-allantoic route.Three day post-inoculation, the allantoic fluid is checked for the NDV growth by spot haemagglutination using 10% chicken RBC.

保存条件：

-20℃

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