| 中文名称: | PD-166866 | ||||
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| 英文名称: | PD-166866 | ||||
| 别名: | 1-[2-Amino-6-(3,5-dimethoxyphenyl)-pyrido[2,3-d]pyrimidin-7-yl]-3-tert-butyl urea | ||||
| CAS No: | 192705-79-6 | 分子式: | C20H24N6O3 | 分子量: | 396.44 |
| CAS No: | 192705-79-6 | ||||
| 分子式: | C20H24N6O3 | ||||
| 分子量: | 396.44 | ||||
基本信息
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产品编号：P10055 |
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产品名称：PD-166866 |
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CAS: |
192705-79-6 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
396.44 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
31mg/mL warmed with 50ºC water bath (78.19mM) |
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Ethanol |
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Water |
Insoluble |
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体内（现配现用） |
4% DMSO+30% PEG 300+2% Tween 80+ddH2O |
4mg/mL |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.5224mL |
12.6122mL |
25.2245mL |
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5mM |
0.5045mL |
2.5224mL |
5.0449mL |
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10mM |
0.2522mL |
1.2612mL |
2.5224mL |
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50mM |
0.0504mL |
0.2522mL |
0.5045mL |
生物活性
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产品描述 |
一种FGF-1受体酪氨酸激酶（FGFR1）的选择性抑制剂，IC50：55nM |
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靶点/IC50 |
FGFR1 52.4nM (IC50) |
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体外研究 |
PD 166866 inhibits human full-length FGFR-1 tyrosine kinase with an IC50 value of 52.4nM and is characterized as an ATP competitive inhibitor of the FGFR-1. PD 166866 is a potent inhibitor of FGFR autophosphorylation in NIH 3T3 cells expressing endogenous FGFR-1 and in L6 cells overexpressing the human FGFR-1 tyrosine kinase. PD 166866 also inhibits bFGF-induced tyrosine phosphorylation of the 44- and 42-kDa (ERK 1/2) mitogen-activated protein kinase isoforms in L6 cells. Daily exposure of PD 166866 to L6 cells at concentrations from 1 to 100nM results in a concentration-related inhibition of bFGFstimulated cell growth for 8 consecutive days with an IC50 value of 24nM. |
推荐实验方法(仅供参考)
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细胞实验: |
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PD 166866 is dissolved in DMSO. PD 166866 or vehicle (0.5% DMSO, final concentration) are added every day to triplicate cultures of cells together with 25ng/mL bFGF to stimulate FGF-driven growth. In some experiments, PD 166866 is added every day to triplicate cultures of cells together with 30ng/mL PDGF-BB to stimulate PDGF-driven growth. Cell number is measured by Coulter counting on days 1, 3, 6 or 8 after drug exposure. |
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动物实验: |
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动物模型 |
Female nude mice |
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剂量 |
20mg/kg |
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给药处理 |
i.p |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
