诺考达唑 - CAS:31430-18-9 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	诺考达唑促销
英文名称:	Nocodazole
别名:	R 17934 R 17934
CAS No:	31430-18-9	分子式:	C₁₄H₁₁N₃O₃S	分子量:	301.32
CAS No:	31430-18-9
分子式:	C₁₄H₁₁N₃O₃S
分子量:	301.32
MDL:	MFCD00005588	EINEC:	250-626-5
EINEC:	250-626-5

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N80005 诺考达唑 ≥99% (psaitong)

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熔点：

300°C

包装规格：

2mg 10mg 50mg in glass bottle

溶解性：

溶于DMSO(10mg/ml 加热）和水

产品描述：

基本信息

产品编号：N80005
产品名称：Nocodazole
CAS:	31430-18-9	储存条件	粉末	2-8℃	四年

分子式：	C14H11N3O3S		溶于液体	-80℃	六个月
分子量	301.32			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	7.5mg/mL (24.89mM)
		Ethanol	Insoluble
		Water	Insoluble
	体内（现配现用）	2% DMSO+30% PEG 300+2% Tween 80+ddH2O	1mg/mL
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.3187mL	16.5937mL	33.1873mL
5mM	0.6637mL	3.3187mL	6.6375mL
10mM	0.3319mL	1.6594mL	3.3187mL

生物活性

产品描述	快速可逆的微管聚合抑制剂，也可有效抑制ABL，ABL(E255K) 和 ABL(T315I) 的活性，IC50 值分别为 0.21?μM，0.53?μM 和 0.64?μM。
靶点/IC₅₀	Abl 91nM (Kd)	ABL(T315I) 170nM (Kd)	EGFR (WT) (Cell-free assay)	BRAF 1.8μM (Kd)
	BRAF(V600E) 1.μM (Kd)	c-KIT 1.6μM (Kd)	MEK1 1.7μM (Kd)	MEK2 1.6μM (Kd)
	MET 1.7μM (Kd)	PI3Kγ 1.5μM (Kd)	Microtubule/Tubulin (Kd)	CRISPR/Cas9 (Kd)
体外研究	Nocodazole exhibits good affinity toward c-KIT, with a Kd value of 1.6 μM in highly malignant human cancer cells. Nocodazole displays good binding affinity toward the components of the mitogen-activated protein kinase (MAPK) pathway, such as BRAF (Kd=1.8μM), BRAF(V600E) (Kd=1.1μM), MEK1 (Kd=1.7μM), and MEK2 (Kd=1.6μM). Nocodazole has the highest affinity for αβIV and the lowest affinity for αβIII. Nocodazole (1nM) induces apoptosis of COLO 205 cancer cells. Nocodazole (≥ 30µg/mL) significantly increases the percentage of annexin-V-binding cells without significantly modifying average forward scatter of human erythrocytes.
体内研究	Nocodazole (5 mg/kg/three times per week, i.p.)has antitumor effects in athymic mice bearing COLO 205 tumor xenografts.Nocodazole (1nM) + R-41400 dramatically increase the levels of p21/CIP1 and p27/KIP1 protein in the tumor tissues.

推荐实验方法(仅供参考)

细胞实验:

Proteins are loaded at 50μg/lane and separated by 12% (w:v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and probed with antibodies for cyclin E, p53, p21/CIP1, p27/KIP1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclin A, cyclin D1, cyclin D3, cyclin B, CDK2, CDK4, and cytochrome C. Immunoreactive bands are visualized by incubating with the colorigenic substrates nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate. The expression of GAPDH is used as the control for equal protein loading.

动物实验:

COLO 205 cells are grown in RPMI 1640 supplemented with 10% FCS. Cells are harvested through two consecutive trypsinizations, centrifuged at 300×g; for 5 min, washed twice, and resuspended in sterile phosphate-buffered saline (PBS). Cells (5×105 ) in 0.1mL are injected subcutaneously between the scapulae of each nude mouse. After transplantation, tumor size is measured with calipers, and the tumor volume is estimated. Once tumors reach a mean size of 200 mm3 , animals receive intraperitoneal injections of DMSO (25μL), R-41400 (50mg/kg), nocodazole (5mg/kg), or R-41400 + nocodazole three times per week for 6 wk.

保存条件：

2-8℃

UN码：

HazardClass：

危害声明：

安全说明：

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