| 中文名称: | ITSA-1 | ||||
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| 英文名称: | ITSA-1 | ||||
| 别名: | ITSA-1 1-(2,4-Dichlorobenzoyl)-1H-benzotriazole | ||||
| CAS No: | 200626-61-5 | 分子式: | C13H7Cl2N3O | 分子量: | 292.12 |
| CAS No: | 200626-61-5 | ||||
| 分子式: | C13H7Cl2N3O | ||||
| 分子量: | 292.12 | ||||
基本信息
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产品编号：I10026 |
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产品名称：ITSA-1 |
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CAS: |
200626-61-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
292.12 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
58mg/mL (198.54mM) |
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Ethanol |
5mg/mL (17.11mM) |
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Water |
Insoluble |
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体内 |
现配现用 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.4233mL |
17.1163mL |
34.2325mL |
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5mM |
0.6847mL |
3.4233mL |
6.8465mL |
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10mM |
0.3423mL |
1.7116mL |
3.4233mL |
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50mM |
0.0685mL |
0.3423mL |
0.6847mL |
生物活性
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产品描述 |
一种TSA（曲古菌素A）抑制剂，通过抑制TSA来活过HDAC。 |
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靶点/IC50 |
HDAC |
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体外研究 |
ITSA1 (50μM; A549 cells) treatment serves to revert the TSA-arrested population to a normal cell cycle distribution. ITSA1 is also able to effect cell cycle rescue over longer duration. ITSA1 (50μM; 5 hours; A549 cells) treatment reduces the number of apoptosis in TSA-treated cells. ITSA1 (50μM; 2 hours; A549 and murine ES cells cells) treatment suppresses TSA-induced histone acetylation. Importantly, suppression of acetylation levels is only observable when ITSA1 is added concurrent with or post TSA treatment. ITSA1 (50μM; 30 minutes; murine ES cells cells) suppresses TSA-activated transcription in murine ES cells. Cell Cycle Analysis |
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Cell Line: |
Murine ES cells |
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Concentration: |
50μM |
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Incubation Time: |
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Result: |
Served to revert the TSA-arrested population to a normal cell cycle distribution. |
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Apoptosis Analysis |
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Cell Line: |
A549 cells |
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Concentration: |
50μM |
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Incubation Time: |
5 hours |
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Result: |
Reduced the number of apoptosis. |
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Western Blot Analysis |
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Cell Line: |
A549 and murine ES cells |
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Concentration: |
50μM |
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Incubation Time: |
2 hours |
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Result: |
Reduced histone acetylation to the baseline level. |
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RT-PCR |
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Cell Line: |
Murine ES cells |
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Concentration: |
50μM |
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Incubation Time: |
30 minutes |
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Result: |
Suppressed TSA-activated transcription. |
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体内研究 |
ITSA-1 (0.5mg/kg; intraperitoneal injection; 3 times/week; for 8 weeks; CBS+/− mice) treatment balances deacetylation activity and suppresses IL-6 and TNF-α expression and thereby attenuated histone acetylationdependent infammatory signaling.[2] |
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Animal Model: |
CBS+/− mice[2] |
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Dosage: |
0.5mg/kg |
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Administration: |
Intraperitoneal injection; 3 times/week; for 8 weeks |
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Result: |
Balanced deacetylation activity and suppressed IL-6 and TNF-α expression. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
