IM-12 - CAS:1129669-05-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	IM-12 促销
英文名称:	IM-12
别名:	IM-12 3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione
CAS No:	1129669-05-1	分子式:	C₂₂H₂₀FN₃O₂	分子量:	377.41
CAS No:	1129669-05-1
分子式:	C₂₂H₂₀FN₃O₂
分子量:	377.41
MDL:	MFCD20527313

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

I10013 IM-12 ≥98%（HPLC） (psaitong)

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包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于(≥9 mg/mL)

产品描述：

基本信息

产品编号：	I10013
产品名称：	IM-12
CAS:	1129669-05-1	储存条件	粉末	2-8℃	四年

分子式：	C22H20FN3O2		溶于液体	-80℃	6个月
分子量：	377.41			-20℃	1个月
化学名：	3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione 3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione
Solubility (25°C)：
体外:	DMSO	75mg/mL (198.72mM)
	Ethanol	10mg/mL warmed with 50ºC water bath (26.49mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(6.62mM);Clear solution
	此⽅案可获得 ≥ 2.5 mg/mL (6.62 mM，饱和度未知) 的澄清溶液。以 1 mL ⼯作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加⼊ 50 μ L Tween-80，混合均匀；然后继续加⼊ 450 μL ⽣理盐⽔定容⾄ 1 mL。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.6496mL	13.2482mL	26.4964mL
5mM	0.5299mL	2.6496mL	5.2993mL
10mM	0.2650mL	1.3248mL	2.6496mL

生物活性

产品描述	一种丝氨酸蛋白激酶（GSK-3β）抑制剂。
靶点	GSK-3β 53nM (IC50)
体外研究	IM-12 inhibits GSK-3β in ReNcell VM cells,with I50 of 3.8μM.IM-12 (3μM) enhances the β-catenin amount,with no further effect at lower or higher concentration.IM-12 (3μM) also attenuates the proliferation of ReNCell VM cells.IM-12 increases TCF-activity of ReNcell VM.
体内研究	Cells are lysed in RIPA buffer,supplemented with protease and phosphatase inhibitors and centrifuged for 5 min at 15,000 rpm.Immunoprecipitation of GSK-3β is performed with a specific mouse monoclonal anti GSK-3β[G8] antibody with 5μg/sample for 2h at 4℃.The bound protein is precipitated with Protein A/G-Plus agarose-beads (10μL beads per sample).GSK-3βkinase activity is measured in a reaction mixture containing final concentrations of:4mM MOPS pH 7.2;0.4mM EDTA;1mM EGTA;2.5mM β-glycerophosphate;4mM MgCl2;40μM BSA;0.05mM DTT.10μg/sample pGS-2 peptide substrate is used.

推荐实验方法(仅供参考)

细胞实验:

To measure viable cells,50-100μL of cell suspension is analyzed using CASY technology with the appropriate program.ReNcell VM cells are seeded at a defined cell number and proliferated for 24h.Then the medium is changed to proliferation medium with added substances at indicated concentrations.The cell number is determined every 24h.Cells are exposed to the added drugs during the whole experiment, whereas the media is changed every 24h.

保存条件：

2-8℃

UN码：

HazardClass：

危害声明：

安全说明：

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