| 中文名称: | (2-羟丙基)-β-环糊精 | ||||
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| 英文名称: | (2-Hydroxypropyl)-β-cyclodextrin | ||||
| 别名: | 羟丙基-β-环糊精 (2-Hydroxypropyl)-β-cyclodextrin | ||||
| CAS No: | 128446-35-5 | 分子式: | C63H112O42 | 分子量: | 1541.54 |
| CAS No: | 128446-35-5 | ||||
| 分子式: | C63H112O42 | ||||
| 分子量: | 1541.54 | ||||
| MDL: | MFCD00074978 | ||||
基本信息
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产品编号： |
H10036 |
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产品名称： |
(2-Hydroxypropyl)-β-cyclodextrin |
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CAS: |
128446-35-5 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
1541.54 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
100mg/mL (64.87mM) |
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Ethanol |
''100mg/mL |
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Water |
100mg/mL (64.87mM) |
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体内(现配现用): |
1.请依序添加每种溶剂：Saline Solubility: 200mg/mL (InfinitymM); Clear solution; Need ultrasonic 2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.08mg/mL (InfinitymM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (InfinitymM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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3.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08mg/mL (InfinitymM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (InfinitymM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 |
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4.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (InfinitymM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (InfinitymM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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生物活性
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产品描述 |
增溶剂和药物赋形剂 蛋白质的保护剂和稳定剂 |
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靶点 |
amyloid-β aggregation |
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体外研究 |
Cell treatment with (2-Hydroxypropyl)-β-cyclodextrin results in the activation of the transcription factor EB, a master regulator of lysosomal function and autophagy, and in enhancement of the cellular autophagic clearance capacity. (2- Hydroxypropyl)-β-cyclodextrin treatment reduces intracellular cholesterol resulting in significant leukemic cell growth inhibition through G2/M cell-cycle arrest and apoptosis. The IC50 values for (2-Hydroxypropyl)-β-cyclodextrin after 72 hours exposure are in the range of 3.86–10.09mM. (2-Hydroxypropyl)-β-cyclodextrin also shows anticancer effects against CML cells expressing a T315I BCR-ABL mutation (that confers resistance to most ABL tyrosine kinase inhibitors), and hypoxiaadapted CML cells that have characteristics of leukemic stem cells. In addition, colony forming ability of human primary AML and CML cells is inhibited by (2-Hydroxypropyl)-β-cyclodextrin |
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体内研究 |
(2-Hydroxypropyl)-β-cyclodextrin administration promotes transcription factor EB-mediated clearance of proteolipid aggregates that accumulate due to inefficient activity of the lysosome-autophagy system in cells derived from a patient with a lysosomal storage disorder. Intraperitoneal injection of (2-Hydroxypropyl)-β-cyclodextrin significantly improves survival in leukemia mouse models. Systemic administration of (2-Hydroxypropyl)-β-cyclodextrin to mice has no significant adverse effects |
推荐实验方法(仅供参考)
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Cell Assay |
Cells are incubated with (2-Hydroxypropyl)-β-cyclodextrin at various concentrations (5, 7.5, 10, 15, 20mM) for 72 hours. Cell viability is assessed using a trypan blue dye exclusion method and cell proliferation is evaluated using a modified methylthiazol-diphenyl- tetrazolium (MTT) assay |
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Animal Administration |
Mice: Mice are intraperitoneally injected with 200μL vehicle (saline) or (2-Hydroxypropyl)-β-cyclodextrin (50 or 150mM) for 20 consecutive days 3 days after transplantation, and survival is monitored daily. Leukemic cell engraftment is confirmed by detection of GFP-positive cells in the recipient’s BM using flow cytometry |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
